Evaluation of miniaturized Illumina DNA preparation protocols for SARS-CoV-2 whole genome sequencing protocol v1

Résumé

This protocol describes the miniaturisation of the Illumina DNA Prep library kit and the use of a rapid modified protocol with fewer clean-up steps and different reagent volumes (full volume, half volume, one-tenth volume). The cost of sequencing can be reduced roughly sevenfold, and the time required to prepare the library can be reduced from 6.5 hours to 3 hours. The protocol adaptation represents a lower-cost, streamlined approach for SARS-CoV-2 sequencing, which can be used to generate genomic data quickly and affordably, particularly in resource-constrained settings.

Citer ce document

Accès au document

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0