Ameliorative effects of Astymin-SN® and vitamin A against X-ray radiation-induced oxidative stress of male Wistar albino rats

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Purpose: To evaluate the ameliorative effects of Astymin-SN® and vitamin A against x-ray radiation-induced oxidative stress in selected organs of albino rats. Methods: Forty rats were assigned to five groups of equal number of rats. Group 1 rats were unirradiated and untreated (control). Group 2 rats were irradiated and untreated. Group 3 rats received 10 ml/kg body weight (b.w) of Astymin-SN® subcutaneously 24-hours before irradiation and administration continued once daily for seven days. Groups 4 and 5 received 100 and 200 mg/kg b.w of vitamin A orally 24-hours before irradiation, and dosing continued once daily for seven days, respectively. All Irradiation groups were exposed to irradiation twice a day and every other day for seven days. A total radiation dose of 7,689.12 μGy was administered to each of the irradiated groups during the seven days. Organs (liver, kidney, and testis) were harvested for tissue homogenate preparations and malondialdehyde (MDA) and the activities of antioxidant enzymes – superoxide dismutase (SOD), glutathione (GPx) and catalase (CAT) were assessed. Results: Marked increase in MDA and significant (p < 0.05) decline in superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the irradiated and untreated group 2 were observed in comparison with the (control) group 1 rats. Remarkably, the mean values of MDA, SOD, CAT, and GPx in the irradiated and treated groups (groups 3, 4 and 5) did not vary significantly from the unirradiated and untreated control. Conclusion: The observed non-significant difference in the assessed parameters between the irradiated and treated groups is evidence of the possible ameliorative potential of Astymin-SN® and vitamin A against x-ray radiation-induced oxidative stress.

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