Concentration technique for Viable but Non-Culturable organisms v1

Résumé

This protocol is based on existing protocols, such as those for resuscitating microbes, freeze-drying, and selective isolation. It merges the parts of the three protocols to create a new protocol for isolating microbes via resuscitation, centrifugation, and selective isolation. The first part involves resuscitating the microbes; the second part subjects the medium to centrifugation (concentration of the microbes); the third and final part involves the selective growth in a selective broth and subsequent growth on selective agar.

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