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Toxoplasma gondii detection and genotyping in the Environmental matrices: soil and water in Gaza, Palestine

Article scientifique 2022 Anglais

Résumé

Abstract The aim of the current study was to determine the prevalence and genotypes of T. gondii oocysts in soil and water samples from Gaza, Palestine. For this purpose290 environmental samples, including 200 from soil and 90 from water, were collected. Soil samples were collected from Wadi-Gaza, vicinity of trash dumpsters, residential home yards, open-air marketplaces, public squares and gardens. Water samples were collected from Wadi-Gaza, wells used for drinking and irrigation. The samples were examined utilizing PCR and Sheather's solution techniques and genotyping of 4 samples was performed by PCR-RFLP. The results revealed that out of the 290 investigated samples, 31 (10.68%) proved contaminated by Sheather’s solution, whereas 22 (7.58%) were positive by PCR. Genotyping results showed that all of the four tested samples belonged to T. gondii Type I. The highest rate of contamination was detected in the samples collected from residential house yards, while public squares and gardens represented the lowest rate. Regarding the water samples, T. gondii oocysts were detected only in the samples collected from Wadi-Gaza. This work confirms the presence of Type I T. gondii in the soil and water of the study area, with soil contamination being heavier than that of water.

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Dardona, Z., Al-Hindi, A., Hafidi, M., Boumezzough, A., Sharif, F., Boussaa, S. (2022). Toxoplasma gondii detection and genotyping in the Environmental matrices: soil and water in Gaza, Palestine. https://doi.org/10.21203/rs.3.rs-1538884/v1

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Auteur(s)

Zuhair Dardona Adnan Al-Hindi Mohamed Hafidi Ali Boumezzough Fadel A. Sharif Samia Boussaa

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