Molecular characterization of the Central Repeat Region of Plasmodium falciparum Circumsporozoite Gene among Selected Malaria Patients in Omdurman, Sudan

Résumé

Abstract Background Malaria is considered one of the most life-threatening diseases due to its raised morbidity and mortality rate, especially in developing countries including Sudan. Plasmodium falciparum remains the most fatal species associated with severe illness as well as high mortality rate. This study aimed to investigate the genetic polymorphism within P. falciparum circumsporozoite gene central repeat region, among selected Sudanese malaria patients. Methods This was a cross-sectional study conducted in Omdurman; Sudan, during the period of Sep 2017 to Feb 2018. Fifty-nine suspected malaria patients were enrolled in this study. Blood samples were collected and screened using rapid diagnostic tests (RDT) and DNA was extracted for Parasite molecular identification using nested PCR (nPCR). Subsequently, PCR positive samples were subjected to central repeat amplification. Results The rapid diagnostic tests detected 78% (46/59) P. falciparum positive cases, 6.8% (4/59) were co-infected with P. falciparum and P. vivax, and 15.3% (9/59) were negative. In contrast, using nPCR showed 44.1% (26/59) patients were P. falciparum positive and 55.9% (33/59) were negative. For the cs central repeat genotyping, only 14 amplicons were obtained with no clear variation in band size. Conclusion The study revealed no clear variation in band sizes, indicating for the absence of variation in repeat length, however, the samples need to be subjected for DNA sequence analysis to identify the most common variants as well as for better understanding of how these variants will effect on the performance of the RTS.S malaria vaccine.

Citer ce document

Accès au document

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0