Accès ouvert

Genetic variability and consequence of Mycobacterium tuberculosis lineage 3 in Kampala-Uganda

Article scientifique 2019 Anglais

Résumé

BACKGROUND: Limited data existed exclusively describing Mycobacterium tuberculosis lineage 3 (MTB-L3), sub-lineages, and clinical manifestations in Kampala, Uganda. This study sought to elucidate the circulating MTB-L3 sub-lineages and their corresponding clinical phenotypes. METHOD: A total of 141 M. tuberculosis isolates were identified as M. tuberculosis lineage 3 using Single nucleotide polymorphism (SNP) marker analysis method. To ascertain the sub-lineages/sub-strains within the M. tuberculosis lineage 3, the direct repeat (DR) loci for all the isolates was examined for sub-lineage specific signatures as described in the SITVIT2 database. The infecting sub-strains were matched with patients' clinical and demographic characteristics to identify any possible association. RESULT: The data showed 3 sub-lineages circulating with CAS 1 Delhi accounting for 55% (77/141), followed by CAS 1-Kili 16% (22/141) and CAS 2/CAS 8% (12/141). Remaining isolates 21% (30/141) were unclassifiable. To explore whether the sub-lineages differ in their ability to cause increased severe disease, we used extent of lung involvement as a proxy for severe disease. Multivariable analysis showed no association between M. tuberculosis lineage 3 sub-lineages with severe disease. The risk factors associated with severe disease include having a positive smear (OR = 9.384; CI 95% = 2.603-33.835), HIV (OR = 0.316; CI 95% = 0.114-0.876), lymphadenitis (OR = 0. 171; CI 95% = 0.034-0.856) and a BCG scar (OR = 0.295; CI 95% = 0.102-0.854). CONCLUSION: In Kampala, Uganda, there are three sub-lineages of M. tuberculosis lineage 3 that cause disease of comparable severity with CAS-Dehli as the most prevalent. Having HIV, lymphadenitis, a BCG scar and a smear negative status is associated with reduced severe disease.

Citer ce document

Wampande, E., Naniima, P., Mupere, E., Kateete, D., Malone, L., Stein, C., Mayanja‐Kizza, H., Gagneux, S., Boom, W., Joloba, M. (2019). Genetic variability and consequence of Mycobacterium tuberculosis lineage 3 in Kampala-Uganda. https://doi.org/10.1371/journal.pone.0221644

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Eddie M. Wampande Peter Naniima Ezekiel Mupere David Patrick Kateete LaShaunda L. Malone Catherine M. Stein Harriet Mayanja‐Kizza Sébastien Gagneux W. Henry Boom Moses Joloba

Statistiques

Consultations : 1

Téléchargements : 0