Environmental impact on Ononis natrix: Phytochemical profiling, heavy metal bioaccumulation, and multifunctional bioactivities
Résumé
Ononis natrix L., a Mediterranean medicinal species traditionally used for its diuretic and anti-inflammatory prope r ties, has recently attracted global scientific interest for its rich profile of bioactive metabolites with potential pharma cological and ecological significance. This study aimed to evaluate the comparative impact of environmental contamin a tion on phytochemical composition, biological activities and heavy metal bioaccumulation in Ononis natrix L. Samples were collected from a polluted site and a non-polluted site in Berezguel (Tebessa, Algeria). The aerial parts were analyzed for heavy metal content: lead (Pb), cadmium (Cd), zinc (Zn), and copper (Cu). Methanolic extracts were prepared from the same plant parts and were analyzed for total phenolic (TPC), flavonoid (TFC), and condensed tannin (TTC) contents. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), phenanthroline, and reducing power. In addition, enzyme inhibitory potential was examined against α-amylase, acetylcholinesterase, and butyrylcholinesterase, while antimicrobial activity was also assessed. Plants from the polluted site bioaccumulated higher levels of Pb, Cd, Zn and Cu. Phytochemical content was also higher in these samples with significantly elevated TPC and TFC. Plants collected from the polluted site exhibited superior antioxidant activity across all assays, with lower IC50 values for ABTS (15.49 ± 1.89 μg/mL) and DPPH (26. 1 ± 0.9 μg/mL) compared to those from the non-polluted site (23. 8 ± 1. 9 and 33. 7 ± 1.9 μg/mL, respectively). Extracts from the polluted site also showed stronger enzyme inhibition, particularly against α-amylase (IC 50 : 268.1 ± 9.6 μg/mL) compared to acarbose (IC 50 : 3177.3 ± 11. 7 μg/mL). Both extracts exhibited high antibacterial activity with the polluted-site extract producing larger inhibition zones for Gram-positive and Gram-negative bacteria. These findings suggest that pollution induced enviro n mental stress enhanced bioactive compound production and therapeutic potential in O. natrix , though metal accumulation in the raw plant raises safety concerns, underscoring the need for standardization and sustainable sourcing strategies.
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