Proteomic profiling of canine osteosarcoma patients

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Abstract Background Canine osteosarcoma (OSA) is an aggressive primary bone tumor in dogs. Metastasis of canine OSA occurs mainly in the lungs through the hematogenous route. Identification of the proteins that are associated with metastasis using proteomic technologies is important not only for the discovery of OSA targets and signaling pathways associated with metastatic OSA but also to provide novel therapeutic targets. The objective of this study was to conduct a proteomic profiling analysis of canine OSA patients. We used mass spectrometry to examine the protein profiles.Results We found the overexpression of five proteins from OSA tissues—myosin (MYO7a), GTPase IMAP family member 5 isoform 1 (GIMAP5), PR domain 15 (PRDM15), A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4), and interleukin receptor type 1 (IL1R1)—and one protein from the serum, rCG38920.Conclusions In this study, we identified numerous proteins in the tissue and serum of patients with malignant and benign OSA or non-OSA through a proteomic approach using mass spectrometry. These proteins may be useful for diagnosis and prognosis, including the prevention of misdiagnosis between malignant OSA and non-OSA as these have similar clinical symptoms in the early stages. In the future, further independent validation of these biomarkers using a greater number of patient samples and different techniques, including Western blot, is necessary to improve diagnostic power for differentiating malignant OSA patients from non-OSA patients.

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