Accès ouvert

Peer Review Report For: Crude oil hydrocarbons' effect on soil microbial metagenome from Niger Delta polluted soils [version 1; peer review: 1 not approved]

Article scientifique 2024 Anglais

Résumé

Crude oil pollution is an endemic environmental problem in the Niger Delta region of Nigeria with background pollution levels greater than the regulatory intervention limit of 5000mg/kg of soil as stipulated in the Environmental Guidelines and Standards for Petroleum Industry in Nigeria (EGASPIN) in most polluted sites. Hence, the essence of this study was to evaluate the extent of damage caused by the pollutant (crude oil hydrocarbons) on the soil physicochemical parameters and microbial communities as well as to determine pollution effects on soil microbial ecosystem services. The soil microbial community composition, diversity, functional genes, and metabolic pathways were studied to evaluate the pollutant effect on soil microbiomes and their ability to utilize petroleum hydrocarbons as carbon and energy sources. Two sites Bodo (N4.620134, 7.282998E) and Ngia-Ama Tombia (N4.9816667; 7.0608333E) were chosen for the study. The America standard testing methods (ASTM International) were used in measuring physicochemical parameters of the soil influencing microbial response and pollutant behavior in the soil environment. Soil pH was measured using a pH meter, total petroleum hydrocarbons (TPH) using gas chromatography with a mass spectrophotometer (GC-MS), polycyclic aromatic hydrocarbons (PAHs) (ASTM D5412-93(2017) e1), Heavy metals analysis (ASTM D8404-21), soil texture (ASTM D6913/D6913M-17). Shotgun metagenomic analysis was used to determine microbial community composition, following “DNA extraction”, library preparation (Nextera ® DNA Flex Library Prep Kit (Illumina, San Diego, CA), and sequencing using Illuminana NovaSeq ® 6000. The results were analyzed using bioinformatics pipelines. The sequences generated were deposited in the European nucleotide Archive (ENA) with project accession number PRJEB53529.

Citer ce document

Okafor, C., Chikere, C., Akaranta, O., Ntushelo, K. (2024). Peer Review Report For: Crude oil hydrocarbons' effect on soil microbial metagenome from Niger Delta polluted soils [version 1; peer review: 1 not approved]. https://doi.org/10.5256/f1000research.137018.r269822

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Chidinma Peace Okafor Chioma Blaise Chikere Onyewuchi Akaranta Khayalethu Ntushelo

Statistiques

Consultations : 1

Téléchargements : 0