Intracellular Cytokine Staining (ICS) Protocol for FACS Analysis v1

Résumé

This protocol provides a comprehensive method for the intracellular staining of lymphocytic cells for Fluorescence-Activated Cell Sorting (FACS) analysis. The procedure outlines the steps for restimulating cells using an OVA/SYRGL peptide or a PMA-Ionomycin control, which is then followed by formaldehyde fixation, saponin-based permeabilization, and targeted antibody staining. Upon completion, users can expect to successfully label specific intracellular cytokines, such as IFN-gamma, TNF-alpha, and IL-2, alongside extracellular markers including CD8 and CD44. These prepared samples will yield distinctly fluorescently labeled cell populations that are optimally prepared for flow cytometric acquisition and subsequent analysis.

Citer ce document

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0