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A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Article scientifique 2017 Anglais

Résumé

Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

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Lloyd, Y., Ngati, E., Salanti, A., Leke, R., Taylor, D. (2017). A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum. https://doi.org/10.1038/s41598-017-13900-4

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Auteur(s)

Yukie Michelle Lloyd Elise Penvadga Ngati Ali Salanti Rose G. F. Leke Diane Wallace Taylor

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