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IL10 (-1082 G>A, rs1800896) gene polymorphisms are associated with oxidative stress in sickle cell disease patients in Uganda

Article scientifique 2025 Anglais

Résumé

BACKGROUND: ) polymorphisms on oxidative stress and cytokine levels in SCD patients from Mulago hospital. METHODS: A case control study with cross-sectional sample size of 163 SCD patients and 189 healthy controls was carried out. The extent of oxidative stress was quantified using Malondialdehyde (MDA) by spectrophotometry. Levels of IL-10 and TNF-α were measured using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The Amplification Refractory Mutation System polymerase chain reaction (ARMS-PCR) assay was used to genotype IL10-1082 A > G, (rs1800896), IL10-819 C > T (rs1800871) and TNF-α-308G > A (rs1800629) and TNF-β + 252 A > G (rs909253) gene polymorphisms. RESULTS: Samples showed significantly (P = 0.0063) higher median plasma levels of MDA in SCD patients (2.756µM) than healthy controls (2.364µM). A similar trend was observed with significantly (P < 0.0001) higher median plasma levels of IL-10 in SCD patients (20.37pg/ml) than healthy controls (7.5pg/ml). The most frequent genotype for IL-10 (-1082, rs1800896) gene polymorphism was heterozygous GA (62.6%). No significant association between IL10 (-1082G > A, rs1800896) gene polymorphisms and SCD was observed (OR = 1.08, 95% CI = 0.54-2.14, P = 0.87). Yet, IL10 homozygous GG (-1082, rs1800896) (22.12pg/ml) that was found to be significantly associated (P = 0.0234) with increased plasma levels of IL-10 as compared to heterozygous genotype (GA) (13.94pg/ml) in SCD patients. Similarly, higher levels of MDA were found to be significantly (P < 0.0001) associated with homozygous GG at IL-10 (-1082, rs1800896). The most frequent and only reported genotype for TNF-α/β gene polymorphisms were heterozygous GA, thus no associations were described. CONCLUSION: In conclusion, our results suggest that the IL10 (-1082 G > A, rs1800896) gene polymorphism is associated with increased oxidative stress and IL-10 cytokine level in Ugandan SCD patients.

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Namuleme, C., Kato, C., Kasozi, D. (2025). IL10 (-1082 G>A, rs1800896) gene polymorphisms are associated with oxidative stress in sickle cell disease patients in Uganda. https://doi.org/10.1186/s40659-025-00639-w

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Auteur(s)

Cissy Namuleme Charles Drago Kato Dennis M. Kasozi

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