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African Journal of Biomedical Research

Article scientifique 2025 Anglais

Résumé

Aim: This study was designed to examine the bacterial diversity and antibiotic resistance profiles of bacterial isolates obtained from borehole and well water in Obudu Urban Area with the goal of evaluating potential public health risks. Methodology and results: A total of 36 water samples were collected from borehole and well waters for three consecutive weeks and was analyzed using standard microbiological techniques. Antibiotic susceptibility testing was done using Kirby Bauer’s disc diffusion method. Multi-drug resistant isolates were selected for 16S Ribosomal RNA gene sequencing for molecular identification. A total of 56 bacterial isolate; Citrobacter spp. (28.6 %), Serratia spp. (17.8%), Proteus spp. (16.2%), Klebsiella spp. (10.7%), Enterobacter spp. (7.1 %), Escherichia coli (5.4%) and Salmonella spp (5.4%) were identified. The results for antibiotic resistance revealed that bacterial isolates were completely resistant to Cefuroxime (100%) with varied resistance to Ceftriaxone (94.64%), Cefotaxime (82.14%), Ceftazidime (80.35%), Meropenem (67.85%), Tetracycline (39.28%), Ciprofloxacin (35.71%), Gentamicin (16.07%), Amikacin (16.07%) and , Cholamphenicol (14.28%). Approximately 19 (34%) of the isolates were multi drug resistance (MDR) to the antibiotics used. Molecular analysis identified Escherichia coli strain KW52, Citrobacter feundii strain VW44, Citrobacter pertucalensis strain JB26, Salmonella enterica strain LB13 and Proteus mirabilis strain JB33 are using the BLAST bioinformatics suite on the NCBI. Conclusion, significance and impact of study: The presence of Antibiotic resistant bacteria phenotypes in drinking water samples emphasizes the importance of microbiological monitoring and effective water treatment strategies. Also, the isolation of opportunistic pathogens and fecal coliforms from this study is a strong indicator of environmental contamination and public health risks.

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Upekiema, A., Okon, E., Ugor, A., Jennifer, A., Nnenna, O., Ndidi, O., Onyebuchi, O. (2025). African Journal of Biomedical Research. https://doi.org/10.53555//ajbr.v27i4s.7127

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Auteur(s)

Adie Francisca Upekiema Ekong Mercy Okon Akobi Peter Ugor Adie Jennifer Onu Euslar Nnenna Ovia Kenneth Ndidi Okafor Collins Onyebuchi

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