Hepatoprotective effect of the methanol fraction of Cuminum cyminum against acetaminophen-induced toxicity in rats
Résumé
Purpose: To evaluate the hepatoprotective activity of methanol fraction of Cuminum cyminum leaves (MFCCL) against acetaminophen (APAP)-induced liver injury in rats, and to characterize its bioactive polyphenolic constituents. Methods: Dried leaves of C. cyminum were extracted with 80 % methanol and partitioned to obtain methanol fraction. Quantitative phytochemical analysis and Gas Chromatography-Flame Ionization Detector (GC-FID) profiling were performed. Acute toxicity was tested in mice (n = 3 per group) using standard methods. For hepatoprotective study, 48 male Wistar rats (150 – 200 g) were randomly assigned to six groups (n = 8). Groups 1 (normal control) and 2 (hepatotoxic control) received distilled water (2 mL/kg). Group 3 received silymarin (200 mg/kg). Groups 4 – 6 received MFCCL (100, 200, and 400 mg/kg, respectively). All treatments were administered orally once daily for seven days, 30 min before daily APAP administration (400 mg/kg, p.o.). On day 8, blood and liver samples were collected for biochemical and histopathological analyses. Results: The MFCCL was rich in flavonoids (53.11 mg/100g) and tannins (3846.67 mg/100g). GC-FID identified isoflavones (25.02 %), flavonones (12.90 %), and gallocatechin (11.86 %) as major polyphenols. Acute toxicity test showed no mortality up to 5000 mg/kg. The serum of APAP-only treated rats exhibited significant (p < 0.05) elevations in liver enzymes, total bilirubin, and hepatic malondialdehyde, with concomitant depletion of antioxidant enzymes and GSH. Pretreatment with MFCCL significantly (p < 0.05) reversed these alterations, restored antioxidant status, and preserved hepatic architecture comparable to silymarin. Conclusion: Methanol fraction of Cuminum cyminum leaves exhibits significant hepatoprotective effect against APAP-induced liver injury, mediated through its polyphenolic constituents and antioxidant mechanisms, supporting its potential as a safe therapeutic agent for drug-induced hepatotoxicity.
Citer ce document
Accès au document
Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter
Voir l'article sur le site de la revueStatistiques
Consultations : 1
Téléchargements : 0