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Modulation of Immunological Responses by Aqueous Extract of Datura Stramonium L. Seeds on Cyclophosphamide-Induced Immunosuppression in Wistar Rats

Article scientifique 2021 Anglais

Résumé

Abstract Background: Datura stramonium L. (Solanaceae) is used traditionally in West Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats.Methods: Following phytochemical analysis of ASEDS and acute toxicity study, thirty Wistar rats of both sexes (180-200 g) were randomized into 6 groups (n = 5). Rats in groups 1, 2 and 3 served as normal, negative and standard controls respectively. Immunosuppression was induced using Cyclophosphamide, 10 mg/kg body weight (b.w.) orally for 27 days. Group 2 rats were untreated while Groups 3-6 rats received 5 mg/kg b.w. Levamisole, 60, 90 and 120 mg/kg b.w. ASEDS orally for 28 days respectively. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of immunosuppressed rats were evaluated.Results: ASEDS indicated LD50 above 5000 mg/kg b.w. with moderate contents of carbohydrates, glycosides, saponins, tannins, terpenoids, and high contents of alkaloids, flavonoids and phenols. Cyclophosphamide triggered significant (p < 0.05) reduction in total leucocyte count and differentials, IgA, IgG, high-density lipoproteins (HDL), catalase, superoxide dismutase, glutathione peroxidase, vitamins A, C and E levels of the negative control rats. Treatment with ASEDS led to significant (p < 0.05) increases in immune cell counts, immunoglobulin synthesis, HDL levels, and antioxidant status of the test groups.Conclusions: The findings demonstrate the immunomodulatory benefits of ASEDS and its potential in the development of potent immunomodulatory drugs.

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Joshua, P., Junaidu, Y., Ekpo, D., Ogidigo, J., Odiba, A., Asomadu, R., Oka, S., Adeniyi, O. (2021). Modulation of Immunological Responses by Aqueous Extract of Datura Stramonium L. Seeds on Cyclophosphamide-Induced Immunosuppression in Wistar Rats. https://doi.org/10.21203/rs.3.rs-738146/v1

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Auteur(s)

Parker Elijah Joshua Yahaya Junaidu Daniel Emmanuel Ekpo Joyce Oloaigbe Ogidigo Arome Solomon Odiba Rita Onyekachukwu Asomadu Samson Ayodeji Oka Olasupo Stephen Adeniyi

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