Alkaline protease from Bacillus cereus was characterized and optimized for eco-friendly degradation of feathers

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Abstract Purpose: To investigate the detailed analysis of alkaline protease production, media optimization, protease assay, mass production, and application in feather degradation by isolates of Bacillus cereus strain isolated from leather industry effluent, Ethiopia.Methods: In this study, media optimization was subjected to nine different parameters like fermentation time, temperature, pH, Substrate concentration, carbon sources, nitrogen sources, metal ions, Inoculum size, and sodium chloride concentration to check the maximum alkaline protease production using Bacillus cereus. Ammonium sulfate and dialysis were used to partially purify the enzyme from Bacillus cereus. SDS-PAGE was used to test the activity and total protein content of the partially purified enzymes, and then extracellular alkaline protease from Bacillus cereus was used to examine hydrolyzed chicken feathers, and the findings were reported.Results: The maximum enzyme activity of crude alkaline protease from Bacillus cereus was achieved at optimized conditions. The specific enzyme activity of partially purified alkaline protease after dialysis (123.35U/mg) showed high activity compared to the crude protease (24.45U/mg). Finally, the partially purified enzyme was tested for its potential to degrade feather waste. Partially purified enzyme demonstrated significant feather degradation of 76.5% on the fifth day at optimized temperature and enzyme concentration.Conclusions: Bacillus cereus was found to cause considerable feather deterioration in this investigation. It's was also been discovered that enzyme activity gets enhanced at a specific optimized condition. Compared to the crude enzyme, partially purified and dialyzed enzymes showed considerable change in the enzyme activity, indicating that they have the potential to break down feathers.

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