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Integrated Laboratory Protocol for Diagnosis of Sexually Transmitted Infections (STIs): ELISA, Rapid Tests, Hemagglutination, and Confirmatory Algorithms v1

Article scientifique 2025 Autre

Résumé

This protocol presents a fully integrated and sequential laboratory workflow for the serological diagnosis of sexually transmitted infections. It describes a combined approach that incorporates rapid immunochromatographic screening for HIV and syphilis, non-treponemal testing using the Rapid Plasma Reagin assay, treponemal confirmation through hemagglutination (TPHA), and enzyme-linked immunosorbent assays (ELISA) for the detection of HIV-1/2, HSV-2, HCV IgG, Chlamydia trachomatis IgG, and HBsAg. When available, automated confirmation using the Abbott Architect/MEIA platform is included to strengthen diagnostic validity. Designed for laboratories operating in low- and middle-income settings, the protocol follows ISO 15189 and CLSI recommendations and outlines all pre-analytical, analytical, and post-analytical requirements necessary to ensure reliable reproducibility in clinical and research environments.

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Ramamonjiarisoa, F., RAZAFIARIMANGA, H., RAMAROSON, D. (2025). Integrated Laboratory Protocol for Diagnosis of Sexually Transmitted Infections (STIs): ELISA, Rapid Tests, Hemagglutination, and Confirmatory Algorithms v1. https://doi.org/10.17504/protocols.io.e6nvw4we2lmk/v1

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Auteur(s)

Faramalala Ramamonjiarisoa HDR Zara Nomentsoa RAZAFIARIMANGA Dr Roseline RAMAROSON

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