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BGISEQ-500 （DNBSEQ-G50）WGS library construction v1

Article scientifique 2020 Anglais

Résumé

BGISEQ-500 is a desktop sequencer developed by BGI. Using DNA nanoball and combinational probe anchor synthesis developed from Complete Genomics™ sequencing technologies, it generates short reads at a large scale. Library construction on the platform includes fragmentation, size selection, end repair and A-tailing, adaptor ligation, PCR amplification, and splint circularization.

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Huang, J., Liang, X., Xuan, Y., Geng, C., Li, Y., Lu, H., Qu, S., Mei, X., Chen, H., Yu, T., Sun, N., Rao, J., Wang, J., Zhang, W., Chen, Y., Liao, S., Jiang, H., Liu, X., Yang, Z., Mu, F., Gao, S. (2020). BGISEQ-500 （DNBSEQ-G50）WGS library construction v1. https://doi.org/10.17504/protocols.io.bim3kc8n

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Auteur(s)

Jie Huang Xinming Liang Yuankai Xuan Chunyu Geng Yuxiang Li Haorong Lu Shoufang Qu Xianglin Mei Hongbo Chen Ting Yu Nan Sun Junhua Rao Jiahao Wang Wenwei Zhang Ying Chen Sha Liao Hui Jiang Xin Liu Z Yang Feng Mu Shangxian Gao

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