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Spatiotemporal control of interferon-induced JAK/STAT signalling and gene transcription by the retromer complex

Article scientifique 2016 Anglais

Résumé

Type-I interferons (IFNs) play a key role in the immune defences against viral and bacterial infections, and in cancer immunosurveillance. We have established that clathrin-dependent endocytosis of the type-I interferon (IFN-α/β) receptor (IFNAR) is required for JAK/STAT signalling. Here we show that the internalized IFNAR1 and IFNAR2 subunits of the IFNAR complex are differentially sorted by the retromer at the early endosome. Binding of the retromer VPS35 subunit to IFNAR2 results in IFNAR2 recycling to the plasma membrane, whereas IFNAR1 is sorted to the lysosome for degradation. Depletion of VPS35 leads to abnormally prolonged residency and association of the IFNAR subunits at the early endosome, resulting in increased activation of STAT1- and IFN-dependent gene transcription. These experimental data establish the retromer complex as a key spatiotemporal regulator of IFNAR endosomal sorting and a new factor in type-I IFN-induced JAK/STAT signalling and gene transcription.

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Chmiest, D., Sharma, N., Zanin, N., Lesegno, C., Shafaq‐Zadah, M., Sibut, V., Dingli, F., Hupé, P., Wilmes, S., Piehler, J., Loew, D., Johannes, L., Schreiber, G., Lamaze, C. (2016). Spatiotemporal control of interferon-induced JAK/STAT signalling and gene transcription by the retromer complex. https://doi.org/10.1038/ncomms13476

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Auteur(s)

Daniela Chmiest Nanaocha Sharma Natacha Zanin Christine Viaris de Lesegno Massiullah Shafaq‐Zadah Vonick Sibut Florent Dingli Philippe Hupé Stephan Wilmes Jacob Piehler Damarys Loew Ludger Johannes Gideon Schreiber Christophe Lamaze

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