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Functional validation of the Plasmodium falciparum K13 C580Y mutation in recently collected Ethiopian isolates

Article scientifique 2026 Anglais

Résumé

Abstract Recent genomic investigation in Ethiopia identified the first detection of the Plasmodium falciparum kelch13 (K13) C580Y substitution in the Horn of Africa. To assess its functional impact, we introduced C580Y into two recently collected Ethiopian clinical isolates using CRISPR-Cas9 genome editing. Ring-stage survival assays showed significantly elevated in vitro dihydroartemisinin (DHA) survival in edited parasites relative to isogenic controls, demonstrating that C580Y confers artemisinin tolerance in contemporary Ethiopian parasite genetic backgrounds.

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Mukherjee, A., Assefa, A., Turlo, C., Needham, L., Shoue, D., Qahash, T., Belachew, M., Tadesse, D., Kassie, E., Berihun, M., Brhane, B., Parr, J., Ferdig, M., Adane, S., Tesfaye, A., Zuromski, J., Fola, A., Tasew, G., Tollera, G., Juliano, J., Bailey, J. (2026). Functional validation of the Plasmodium falciparum K13 C580Y mutation in recently collected Ethiopian isolates. https://doi.org/10.64898/2026.03.17.712112

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Auteur(s)

Angana Mukherjee Ashenafi Assefa Christopher V. Turlo Lisa Checkley Needham Douglas A. Shoue Tarrick Qahash Mahlet Belachew Dagimawie Tadesse Enirsie Kassie Mulu Berihun Bokretsion G. Brhane Jonathan B. Parr Michael T. Ferdig Sisay Adane Adisu Tesfaye Jenna Zuromski Abebe A. Fola Geremew Tasew Getachew Tollera Jonathan J. Juliano Jeffrey A. Bailey

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