Study of Toxic Profile of Staphylococcus Aureus Isolated From Raw Milk Samples of Mastitis SOR Consumption in Oran Area, Algeria

Résumé

Mastitis S. aureus is considered one of the major diseases in dairy cattle. The presence of S. aureus causes damage to the mammary gland tissue that will impact milk production in both volume and quality. The many virulence factors such as toxins and proteins or surface polysaccharides are involved in the pathology. This study determines the toxic profile of S. aureus strains isolated from raw milk samples from bovine mastitis diagnosed in dairy farms in Oran. After identification by MALDI-TOF, spa typing and MLST have identified the phylogenetic position of the strains. Search for virulence factors is performed by real-time PCR TaqMan type, to detect manipulation in one of the four genes of virulence factors of S. aureus: tst for TSST-1, pvl leucocidin for ED, sea for enterotoxin A, seb for enterotoxin B, in this study sought. Toxins research shows that only a few strains have proven carriers of different virulence genes including the gene encoding the pvl Panton-Valentine leukocidin. Other strains were positive for the presence of tst gene encoding the toxin of toxic shock syndrome (TSST-1), 100% of the Staphylococcus aureus isolates identified were sensitive to methicillin. In conclusion, the results obtained in this work have determined the phylogenetic profile, toxic and sensitivity profile of the méticiline strains studied. Stem found produisantes as the Panton-Valentine leukocidin are sensitive to méticilline. Cette study is the first molecular characterization study of animal strains of S. aureus isolated in the region of Oran.

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