Addition of monophosphoryl lipid A (MPLA) to Toxoplasma ROP47 DNA

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Abstract We constructed a ROP47 DNA vaccine combined with monophosphoryl lipid A (MPLA) and used it to immunize mice. Five groups of mice were injected every 2 weeks with PBS, empty vector, MPLA, pROP47, or pROP47 with MPLA. All mice were injected intramuscularly three times. Blood samples from all experimental mice were collected from the tail vein at 2, 4, 6, and 10 weeks after the first injection. The levels of IgG, IgG1, IgG2a, IFN-γ, IL-4, IL-10, and IL-12 in blood from all experimental mice were measured. The mice were challenged intragastrically with 20 cysts of Toxoplasma gondii PRU strain 2 weeks after the last vaccination. The mice immunized with MPLA/pROP47 produced higher levels of IgG, IgG2a, IFN-γ, and IL-12 compared with other groups. Furthermore, after a challenge with 20 cysts, higher numbers of cysts were present in the brains of other experimental mice compared with MPLA/pROP47-immunized mice. The pROP47 vaccine markedly stimulated humoral and cellular immune responses in immunized mice and MPLA enhanced the immune protection induced by the pROP47 DNA vaccine.

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