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Culture of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; f.2019-nCoV) v1

Article scientifique 2020 Anglais

Résumé

We briefly describe a method to inoculate a susceptible cell line with a human patient sample in order to culture the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease, COVID-19. Clinical samples including nasopharyngeal swabs and aspirates were inoculated onto confluent monolayers of African green monkey kidney Vero C1008, clone E6 cells (ATCC®-CRL-1586) grown in Opti-Mem reduced serum growth medium supplemented with 3% foetal bovine serum in polystyrene, flat-sided, screw-cap 3 mL cell culture tubes. This work was conducted in Queensland under PC3 laboratory conditions by experienced scientists.

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Pyke, A., Mackay, I., Moore, F., Hurk, A., Northill, J., Finger, M., Simpson, N., Nair, N., Burtonclay, P., Moore, P., Wheatley, S., Moody, S., Hall‐Mendelin, S., Gamez, E., Jong, A., Huang, B., Taylor, C., Warrilow, D., Genge, D., Hewitson, G., Sultana, I., McMahon, J., Barcelon, J. (2020). Culture of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; f.2019-nCoV) v1. https://doi.org/10.17504/protocols.io.bcduis6w

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Auteur(s)

Alyssa T. Pyke Ian M Mackay Frederick Moore Andrew F. van den Hurk Judy A Northill Mitchell Finger Natalie Simpson Neelima Nair Peter Burtonclay Peter F. Moore Sarah Wheatley Sean Moody Sonja Hall‐Mendelin Elisabeth Gamez Amanda De Jong Ben Huang Carmel Taylor David Warrilow Doris Genge Glen Hewitson Inga Sultana Jamie McMahon Jean Barcelon

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