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Partial characterization of Prunus Necrotic Ringspot Virus on apple in EGYPT

Article scientifique 2021 Anglais

Résumé

Abstract A severe isolate of Prunus necrotic ringspot virus (PNRSV) was isolated from apple orchards in the vicinity of Nubaria city, Beheira governorate, Egypt. Infected-apple trees showed chlorotic, necrotic ringspots, and shoot holes on leaves. Severely infected- trees withered, became useless, and were removed causing severe economic losses. Reverse transcriptase (RT) polymerase chain reaction (PCR), RT-PCR, using degenerate primer pair for the coat protein (CP) gene of Ilarvirus amplified products similar to those produced from peach and apricot isolates of PNRSV-infecting stone fruits). Dot blotting immuno-binding assay (DBIA showed a positive reaction between PNRSV-infected apple sap and an Egyptian antiserum for PNRSV. Purified preparation from infected leaves, using the electro-elution technique yielded nucleoprotein, which had Amax and Amin at 260 and 240 nm respectively. Electron microscopy examination showed spherical virions with ca. 26 nm in diameter.

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Abdel-Salam, A., Mokbel, S. (2021). Partial characterization of Prunus Necrotic Ringspot Virus on apple in EGYPT. https://doi.org/10.21203/rs.3.rs-364526/v1

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Aly M. Abdel-Salam Samah Mokbel

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