Accès ouvert

Super high-resolution single-molecule sequence-based typing of HLA class I alleles in HIV-1 infected individuals in Ghana

Article scientifique 2022 Anglais

Résumé

Polymorphisms in human leukocyte antigen (HLA) class I loci are known to have a great impact on disease progression in HIV-1 infection. Prevailing HIV-1 subtypes and HLA genotype distribution are different all over the world, and the HIV-1 and host HLA interaction could be specific to individual areas. Data on the HIV-1 and HLA interaction have been accumulated in HIV-1 subtype B- and C-predominant populations but not fully obtained in West Africa where HIV-1 subtype CRF02_AG is predominant. In the present study, to obtain accurate HLA typing data for analysis of HLA association with disease progression in HIV-1 infection in West African populations, HLA class I (HLA-A, -B, and -C) four-digit allele typing was performed in treatment-naïve HIV-1 infected individuals in Ghana (n = 324) by a super high-resolution single-molecule sequence-based typing (SS-SBT) using next-generation sequencing. Comparison of the SS-SBT-based data with those obtained by a conventional sequencing-based typing (SBT) revealed incorrect assignment of several alleles by SBT. Indeed, HLA-A*23:17, HLA-B*07:06, HLA-C*07:18, and HLA-C*18:02 whose allele frequencies were 2.5%, 0.9%, 4.3%, and 3.7%, respectively, were not determined by SBT. Several HLA alleles were associated with clinical markers, viral load and CD4+ T-cell count. Of note, the impact of HLA-B*57:03 and HLA-B*58:01, known as protective alleles against HIV-1 subtype B and C infection, on clinical markers was not observed in our cohort. This study for the first time presents SS-SBT-based four-digit typing data on HLA-A, -B, and -C alleles in Ghana, describing impact of HLA on viral load and CD4 count in HIV-1 infection. Accumulation of these data would facilitate high-resolution HLA genotyping, contributing to our understanding of the HIV-1 and host HLA interaction in Ghana, West Africa.

Citer ce document

Nii-Trebi, N., Matsuoka, S., Kawana‐Tachikawa, A., Bonney, E., Abana, C., Ofori, S., Mizutani, T., Ishizaka, A., Shiino, T., Ohashi, J., Naruse, T., Kimura, A., Kiyono, H., Ishikawa, K., Ampofo, W., Matano, T. (2022). Super high-resolution single-molecule sequence-based typing of HLA class I alleles in HIV-1 infected individuals in Ghana. https://doi.org/10.1371/journal.pone.0269390

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Nicholas Israel Nii-Trebi Saori Matsuoka Ai Kawana‐Tachikawa Evelyn Yayra Bonney Christopher Zaab-Yen Abana Sampson B. Ofori Taketoshi Mizutani Aya Ishizaka Teiichiro Shiino Jun Ohashi Taeko K. Naruse Akinori Kimura Hiroshi Kiyono Koichi Ishikawa William Ampofo Tetsuro Matano

Statistiques

Consultations : 1

Téléchargements : 0