Accès ouvert

The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues

Article scientifique 2016 Anglais

Résumé

The extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7% (95% CI: 78.1-99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis.

Citer ce document

García‐Basteiro, A., Ismail, M., Carrilho, C., Ussene, E., Castillo, P., Chitsungo, D., Rodríguez, C., Lovane, L., Vergara, A., López‐Varela, E., Mandomando, I., Lorenzoni, C., Ordï, J., Menéndez, C., Bassat, Q., Martínez, M. (2016). The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues. https://doi.org/10.1038/srep20703

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Alberto L. García‐Basteiro Mamudo R. Ismail Carla Carrilho Esperança Ussene Paola Castillo Dércio Chitsungo Cristina Leal Rodríguez Lucília Lovane Andrea Vergara Elisa López‐Varela Inácio Mandomando Cesaltina Lorenzoni Jaume Ordï Clara Menéndez Quique Bassat Miguel J. Martínez

Statistiques

Consultations : 3

Téléchargements : 0