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First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso

Article scientifique 2022 Anglais

Résumé

BACKGROUND: Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-α-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso. METHODS: Antifungals susceptibility were performed using Kirby-Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole. RESULTS: Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles. CONCLUSIONS: This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis.

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Dovo, E., Zohoncon, T., Tovo, S., Soubeïga, S., Kiendrebeogo, I., Yonli, A., Ouédraogo, R., Dabiré, A., Djigma, F., Nadembega, C., Belemgnegre, M., Ouédraogo, P., Obiri‐Yeboah, D., Simporè, J. (2022). First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso. https://doi.org/10.1186/s12879-022-07619-5

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Essi Etonam Dovo Théodora Mahoukèdè Zohoncon S Tovo Serge Théophile Soubeïga Isabelle Touwendpoulimdé Kiendrebeogo Albert Théophane Yonli Rogomenoma Alice Ouédraogo Amana Mètuor Dabiré Florencia Wendkuuni Djigma Christelle Nadembega Marius Belemgnegre Paul Ouédraogo Dorcas Obiri‐Yeboah Jacques Simporè

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