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Heterogeneous loss of HIV transcription and proviral DNA from 8E5/LAV lymphoblastic leukemia cells revealed by RNA FISH:FLOW analyses

Article scientifique 2016 Anglais

Résumé

8E5/LAV cells harbor a single HIV provirus, and are used frequently to generate standards for HIV genome quantification. Using flow cytometry-based in situ mRNA hybridization validated by qPCR, we find that different batches of 8E5 cells contain varying numbers of cells lacking viral mRNA and/or viral genomes. These findings raise concerns for studies employing 8E5 cells for quantitation, and highlight the value of mRNA FISH and flow cytometry in the detection and enumeration of HIV-positive cells.

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Wilburn, K., Mwandumba, H., Jambo, K., Boliar, S., Solouki, S., Russell, D., Gludish, D. (2016). Heterogeneous loss of HIV transcription and proviral DNA from 8E5/LAV lymphoblastic leukemia cells revealed by RNA FISH:FLOW analyses. https://doi.org/10.1186/s12977-016-0289-2

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Auteur(s)

Kaley M. Wilburn Henry C. Mwandumba Kondwani Jambo Saikat Boliar Sabrina Solouki David G. Russell David W. Gludish

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