Biosynthesis of selenium nanoparticles from Dahlia pinnata tuberous roots with antibacterial, antidiabetic, and erythrocyte membrane protective activities

Résumé

Abstract Selenium nanoparticles (SeNPs) synthesized using Dahlia pinnata L tuber extract exhibit significant antimicrobial, antidiabetic, and anti-inflammatory properties. This study describes the green synthesis of SeNPs using D. pinnata L. tuber extract, a sustainable approach that leverages plant-based compounds. Green synthesis was confirmed via UV-Vis spectroscopy (280 nm peak), XRD trigonal crystal structure with planes (100), (101), (110), (111), and (201), and TEM (spherical particles, 17.37 nm average size). FTIR revealed functional groups (C–H, C = O, Se–Se), while HPLC identified gallic acid (1070.58 µg/g) and chlorogenic acid (903.87 µg/g). In antimicrobial studies, SeNPs demonstrated strong efficacy against 70 Escherichia coli isolates from laboratory samples of diabetic patients. For 15 multidrug-resistant (MDR, 21.4%) isolates, SeNPs had MICs of 25–50 µg/ml (mean: 35 ± 12 µg/ml) and MBCs of 50–100 µg/ml (mean: 76.6 ± 26 µg/ml). Non-MDR isolates (78.6%) were more sensitive, with MICs of 10–25 µg/ml (mean: 15 ± 4.5 µg/ml) and MBCs of 25–50 µg/ml (mean: 35 ± 12 µg/ml), outperforming selenium precursors and vancomycin. SeNPs also showed antidiabetic potential through α-amylase inhibition (IC 50 = 50.32 µg/ml) and α-glucosidase inhibition (IC 50 = 31.55 µg/ml), though less effective than acarbose (IC 50 = 5.85 µg/ml and 3.93 µg/ml, respectively). In anti-inflammatory assays, SeNPs achieved dose-dependent hemolysis inhibition, with 96.0% inhibition at 1000 µg/ml and an IC 50 of 11.53 µg/ml, compared to indomethacin’s IC 50 of 4.51 µg/ml. These findings demonstrate the promising in vitro bioactivities of SeNPs synthesized from D. pinnata L. tuber extract and support their potential for further preclinical investigation.

Citer ce document

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0