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Novel coronavirus (2019-nCoV) real-time RT-PCR ORF1ab 2020 (Wuhan-ORF1ab; nCoV-specific test) v2

Article scientifique 2020 Anglais

Résumé

A real-time RT-PCR to detect the "novel Wuhan" betacoronavirus or Wuhan seafood market pneumonia virus. Based on sequence MN908947 made available by Professor Yong-Zhen Zhang, Fudan University, Shanghai, China. The target region is within the ORF1ab sequence. Not tested on wild-type virus (as of 25Jan2020), it is expected to be capable of only detecting the Wuhan coronavirus. ·Limit of detection not yet determined. Notes Assay is fully optimised (as of 24Jan2020). A final name for this virus has not been decided (as of 25Jan2020).

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Northill, J., Mackay, I. (2020). Novel coronavirus (2019-nCoV) real-time RT-PCR ORF1ab 2020 (Wuhan-ORF1ab; nCoV-specific test) v2. https://doi.org/10.17504/protocols.io.bb3qiqmw

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Auteur(s)

Judy A Northill Ian M Mackay

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