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Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study

Article scientifique 2022 Anglais

Résumé

OBJECTIVE: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. RESULTS: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing.

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Leta, D., Gutema, G., Hagos, G., Diriba, R., Bulti, G., Sura, T., Ayana, D., Chala, D., Lenjiso, B., Bulti, J., Abdella, S., Tola, H. (2022). Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study. https://doi.org/10.1186/s13104-022-06184-z

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Auteur(s)

Dereje Leta Gadissa Gutema Gebremedhin Gebremichael Hagos Regasa Diriba Gutema Bulti Tolawak Sura Desta Ayana Dawit Chala Boki Lenjiso Jaleta Bulti Saro Abdella Habteyes Hailu Tola

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