Dysregulated Circulating micro RNAs Markers: New Evidence into Expression Pattern in Children with T1D among Egyptian Population

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Abstract Background: miRNAs are gaining access as promising markers for a variety of autoimmune disorders yet, deviations between individuals at risk or developed T1D remains to be thoroughly explored. Objective: to study the pattern of miRNA expression profiling in plasma obtained from patients with T1D and the matched control subjectsPatients and Methods: equally divided numbers of T1D patients (90) and apparently healthy-matched control children (90) were analyzed for their expression profile of plasma miRNAs; miR-101-5p, 146-5p, 21-5p, miR-375, miR-126, and Let7a-5p by reverse transcriptase (RT-PCR) through quantitative real time technique.Results: the two studied groups were significantly different in respect to their biochemical parameters; FBG, 2hpp and HbA1c levels (p<0.05). Among the deregulated molecules, miR-101, miR-21 and-375 were highly expressed, whereas, miR-146-5p, miR-126 and miR-Let7a-5p showed significant low levels of expression in patients compared to control subjects (p<0.05). MiR-101, miR-146 were significantly correlated to age at diagnosis of T1D and disease duration respectively. Furthermore, miR-126, -Let7a-5p showed significant negative correlation with meanA1C values, the matter that was confined by multivariate analysis.Conclusion: dysregulation of the analyzed six micro RNAs pointed out to their pivotal role to be important biomarkers for T1D development.

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