Acidic culture medium enhances secretion of myoglobin and GDF-15 in MCF-7 breast cancer cells– multiplex assay data

Résumé

Abstract Metastatic breast cancer is one of the leading causes of cancer mortality worldwide. While many therapeutic strategies try to control and combat this disease, a better understanding of cellular communication within the acidic tumour microenvironment is needed to improve treatment success. Here we characterized the secretory profile of MCF-7 breast cancer cells in medium of physiological pH, and compared it to those cells treated with medium of 6.5 and 3.2. MCF-7 cells secreted IP-10, VEGF, EGF, FGF, G-CSF, GDF-15, and myoglobin at baseline. When treated with acidic medium, MCF-7 cells downregulated secretion of many of these factors, although myoglobin and GDF-15 were significantly increased at pH 6.5 (1hr) (p < 0.0001). This may provide a survival advantage to the tumour cells at slightly acidic pH. Further studies investigating the effects of acidosis on breast carcinoma cells are needed to understand both their autocrine and paracrine signaling functions in the tumour microenvironment.

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