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Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces

Article scientifique 2024 Anglais

Résumé

Tuberculosis (TB) is the top cause of death from a single infectious pathogen after COVID-19. Despite molecular diagnostic advances, two-thirds of the 10 million annual TB cases are still diagnosed using direct smear microscopy which has ~50% sensitivity. To increase the analytical performance of smear microscopy, we developed and characterized a novel polymer (Polydiallyldimethylammonium chloride [PDADMAC]) engraftment on inexpensive polystyrene (PS) specifically functionalized for mycobacterial capture. Engraftment is achieved via UV photopolymerization of DADMAC monomer on plasma-activated PS. The platform was tested on sputum from presumptive TB cases in Kampala, Uganda (n = 50), with an increased overall sensitivity of 81.8% (27/33) vs. fluorescent smear microscopy 57% (19/33) compared to a molecular (Cepheid GeneXpert MTB/RIF) gold standard. Frugal smear diagnostic innovation that is rapid and does not require dedicated instrumentation may offer an important solution to bridge the TB diagnostic gap.

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Jiang, X., Mathekga, B., Singh, D., Coon, D., Sinha, A., Armstrong, D., Acharya, S., Mao, H., Manabe, Y. (2024). Selective mycobacterial capture with ultraviolet-polymerized poly-dimethyldiallyl chloride functionalized surfaces. https://doi.org/10.1007/s10856-024-06829-4

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Auteur(s)

Xuesong Jiang Bonolo S.P. Mathekga Digvijay Singh Devin Coon Anjana Sinha Derek T. Armstrong Soumyadipta Acharya Hai‐Quan Mao Yukari C. Manabe

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