Assessment of Pyrethroid Insecticides Susceptibility Profiles and Molecular Evaluation of L1014F kdr mutant alleles in Populations of Culex quinquefasciatus from Lymphatic Filariasis Endemic Communities in Akwa Ibom State, Nigeria

Résumé

Abstract Background: This study investigated the dynamics in pyrethriod resistance and the presence/frequencies of L1014F knockdown resistance mutant allelles in Culex quinquefasciatus vector populations from Uruan Local Government Area of Akwa Ibom State, Nigeria. Methods: Centre for Disease Control (CDC) bioassay was used to evaluate resistance to permethrin, deltamethrin and alphacypermethrin insecticides following standard protocols. Allele-Specific Polymerase Chain Reaction (AS-PCR) was used in detecting target site insensitivity in the vector populations. Results: Results obtained revealed that vectors from all the study sites were resistant to permethrin insecticide (mortality rate: 18% - 23%). Suspected resistance (mortality rate: 90% - 93%) to deltamethrin and low resistance (mortality rate: 82% - 85%) to alphacypermethrin insecticides were detected. knockdown was more rapid with deltamethrin and alphacypermethrin than with permethrin across the study sites considering their KDT50 and KDT95. The frequency of the resistant phenotypes ranged between 35.14 % to 55.3 % across the study sites with a net of 45.1 % resistant phenotype recorded in this study. The 1014F allelic frequency calculated from Hardy-Weinberg principle for vector populations across the study sites ranged from 0.5 (50 %) to 0.7763 (77.63 %). All populations witnessed significant (p < 0.05) deviations from Hardy-Weinberg equilibrium in the distribution of these alleles. Conclusion: The findings of this study show that there is a tendency to record an entire population of resistant vectors in this study area over time due to natural selection.

Citer ce document

Accès au document

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0