Accès ouvert

Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm

Article scientifique 2019 Anglais

Résumé

Abstract Stem rust (caused byPuccinia graminisf. sp.tritici) is a major disease of wheat. To understand the genetic basis of stem rust resistance in Nebraska winter wheat, a set of 330 genotypes representing two nurseries (DUP2015 and TRP2015) were evaluated for resistance to a Nebraska stem rust race (QFCSC) in two replications. The TRP2015 nursery was also evaluated for its resistance to an additional 13 stem rust races. The analysis of variance revealed significant variation among genotypes in both populations for stem rust resistance. Nine stem rust genes,Sr6,Sr31,Sr1RSAmigo,Sr24,Sr36,SrTmp,Sr7b,Sr9b, andSr38, were expected and genotyped using gene-specific markers. The results of genetic analysis confirmed the presence of seven stem rust resistance genes. One genotype (NE15680) contained targetalleles for five stem rust resistance genes and had a high level of stem rust resistance against different races. Single marker analysis indicated thatSr24andSr38were highly significantly associated with stem rust resistance in the DUP2015 and TRP2015 nurseries, respectively. Linkage disequilibrium analysis identified the presence of 17 SNPs in high linkage with theSr38-specific marker. These SNPs potentially tagging theSr38gene could be used in marker-assisted selection after validating them in additional genetic backgrounds.

Citer ce document

Mourad, A., Sallam, A., Belamkar, V., Wegulo, S., Bai, G., Mahdy, E., Bakheit, B., El-Wafa, A., Jin, Y., Baenziger, P. (2019). Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm. https://doi.org/10.1038/s41598-019-47986-9

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Amira M. I. Mourad Ahmed Sallam Vikas Belamkar Stephen N. Wegulo Guihua Bai Ezzat Mahdy B. R. Bakheit Atif Abo El-Wafa Yue Jin P. Stephen Baenziger

Statistiques

Consultations : 1

Téléchargements : 0