Silver Nanoparticles mediated-marine Fungal Amylase improving Dicentrarchus labrax larvae growth performance

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Abstract Background Biosynthesis of nanoparticles has fueled using materials to produce different metallic nanoforms for aquaculture applications. This study investigates the capability of marine Aspergillus flavus amylase (Amy) to synthesize silver nanoparticles (AgNPs) and studied its impact on Dicentrarchus labrax (sea bass) larvae growth performance. Characterization of Amy-AgNPs was carried out by FTIR, UV–Vis’s spectroscopy and SEM. The effect of Amy-AgNPs for antimicrobial and antioxidant were studied.Results The colloidal brown color of Amy-AgNPs with FTIR analysis indicated that amylases were responsible for the capping of the nanoparticles and made it more stable. Spherical Amy-AgNPs had size of 22.88 to 26.35 nm. Significantly, Amy-AgNPs inhibited the growth of fish tested microbes by maximum zone of inhibition 13-20 mm and maximum MICs ranged from 1.6 to 6.3 µg/ml. Also, it acted as scavenged DPPH by 31.46 to 94.0 %. Sea bass larval morphometric measurements including: mean total length (TL), width (W), total weight (TW), larvae survival percentage (%) and specific growth rate (SGR) were recorded, compared to the control group using different concentrations (1, 5, 10, 20%) of encapsulated Amy-AgNPs during larval feeding regime for a period of four weeks. The results revealed that 5% Amy-AgNPs was significantly the most effective concentration at P < 0.05 that achieved higher survival percentages; 51±2.8% and increasing the specific growth rate by 16.23±0.62%. Conclusion This work concluded the effectiveness of marine fungal amylase to synthesize AgNPs, and it is the first record using Amy-AgNPs to reduce the fish larval mortality by protecting and improving its performance.

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