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Protein expression of Myt272-3 recombinant clone and in silico prediction of a possible vaccine candidate against Mycobacterium tuberculosis

Article scientifique 2016 Anglais

Résumé

Purpose: To investigate the expression of Myt272-3 recombinant protein and also to predict a possible protein vaccine candidate against Mycobacterium tuberculosis.Methods: Myt272-3 protein was expressed in pET30a+-Myt272-3 clone. The purity of the protein was determined using Dynabeads® His-Tag Isolation & Pulldown. Protein sequence was analysed in silico using bioinformatics software for the prediction of allergenicity, antigenicity, MHC-I and MHC-II binding, and B-cell epitope binding.Results: The candidate protein was a non-allergen with 15.19 % positive predictive value. It was also predicted to be antigenic, with binding affinity to MHC-I and MHC-II, as well as B-cell epitope binding.Conclusion: The predicted results obtained in this study provide a guide for practical design of a new tuberculosis vaccine.Keywords: Vaccine, Mycobacterium tuberculosis, Epitope prediction, Histocompatibility complexes

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Usman, M., Ismail, S., Teoh, T. (2016). Protein expression of Myt272-3 recombinant clone and in silico prediction of a possible vaccine candidate against Mycobacterium tuberculosis. https://doi.org/10.4314/tjpr.v15i7.7

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Auteur(s)

Mohammed Maikudi Usman Salmah Ismail Teow Chong Teoh

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