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Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Article scientifique 2017 Anglais

Résumé

AIM: Enteritidis (SE) specific antibodies in chicken sera. MATERIALS AND METHODS: protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device. RESULTS: antigen were 88.8%, 90%, and 89%, respectively. CONCLUSION: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.

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Ibrahim, H., Sayed, R., Abdel-Aziz, W., Soliman, R. (2017). Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks. https://doi.org/10.14202/vetworld.2017.848-853

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Auteur(s)

Hazem Mohammed Ibrahim Rafik Hamed Sayed Wafaa Ragab Abdel-Aziz Rafik Soliman

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