Accès ouvert

RTS,S/AS01E Malaria Vaccine Induces Memory and Polyfunctional T Cell Responses in a Pediatric African Phase III Trial

Article scientifique 2017 Anglais

Résumé

Comprehensive assessment of cellular responses to the RTS,S/AS01E vaccine is needed to understand potential correlates and ultimately mechanisms of protection against malaria disease. Cellular responses recognizing the RTS,S/AS01E-containing circumsporozoite protein (CSP) and Hepatitis B surface antigen (HBsAg) were assessed before and one month after primary vaccination by intracellular cytokine staining and 16-color flow cytometry in 105 RTS,S/AS01-vaccinated and 74 rabies-vaccinated participants (controls) in a pediatric phase III trial in Africa. RTS,S/AS01E-vaccinated children had significantly higher frequencies of CSP- and HBsAg-specific CD4+ T cells producing IL-2, TNF-α and CD40L and HBsAg-specific CD4+ T producing IFN-γ and IL-17 than baseline and the control group frequencies. Vaccine-induced responses were identified in both central and effector memory compartments. Effector memory CD4+ T cells expressing IL-4 and IL-21 were detected recognizing both vaccine antigens. Consistently higher response rates to both antigens in RTS,S/AS01E-vaccinated than comparator-vaccinated children were observed. RTS,S/AS01E induced polyfunctional CSP- and HBsAg-specific CD4+ T cells, with a greater degree of polyfunctionality in HBsAg responses. In conclusion, RTS,S/AS01E vaccine induces T cells of higher quality and complexityfunctional heterogeneity and polyfunctionality than previously characterized. Responses detected in memory CD4+ T cell compartments may provide correlates of RTS,S/AS01-induced immunity and duration of protection in future correlates of immunity studies.

Citer ce document

Moncunill, G., Rosa, S., Ayestaran, A., Nhabomba, A., Mpina, M., Cohen, K., Jairoce, C., Rutishauser, T., Campo, J., Harezlak, J., Sanz, H., Díez‐Padrisa, N., Williams, N., Morris, D., Aponte, J., Valim, C., Daubenberger, C., Dobaño, C., McElrath, M. (2017). RTS,S/AS01E Malaria Vaccine Induces Memory and Polyfunctional T Cell Responses in a Pediatric African Phase III Trial. https://doi.org/10.3389/fimmu.2017.01008

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Gemma Moncunill Stephen C. De Rosa Aintzane Ayestaran Augusto Nhabomba Maxmillian Mpina Kristen W. Cohen Chenjerai Jairoce Tobias Rutishauser Joseph J. Campo Jaroslaw Harezlak Héctor Sanz Núria Díez‐Padrisa Nana Aba Williams Daryl E. Morris John J. Aponte Clarissa Valim Claudia Daubenberger Carlota Dobaño M. Juliana McElrath

Statistiques

Consultations : 2

Téléchargements : 0