Root-Derived Phytochemicals from Inula confertiflora for Antioxidant and Antibacterial Activities

Résumé

Inula confertiflora, a medicinal herb indigenous to Ethiopia, often used in traditional treatments for inflammatory and pain-related conditions. The root of I. confertiflora was soaked with n-hexane, ethyl ether, and acetone solvents. The proportion of crude extracts derived from ethyl ether extracts was 1.4 times higher than that of n-hexane and 1.2 times higher than that of acetone. Analytical detection results of crude extracts confirmed that I. confertiflora contained a variety of different preliminary phytochemicals. The higher concentrations of total flavonoids and other polar phytoconstituents present in the ethyl ether extracts were associated with greater radical-scavenging effectiveness in the DPPH solution. More than half of the bacterial growth efficiency was restricted by phytochemicals derived from I. confertiflora root extracts using non-polar, medium-polar, and high-polar solvents. It is crucial to note that medium-polar extracts of I. confertiflora root decreased the inhibitory effect on the development of both gram-positive and gram-negative bacterial strains at a concentration of 100 μg/mL using acetone as the solvent. Additionally, they exhibited improved radical scavenging against a DPPH solution. Moreover, molecular docking simulation clearly revealed that I. confertiflora extracts have a strong binding affinity toward four key bacterial target proteins (LasB (-7.9kcal/mol), PBP2a (-8.0kcal/mol), FabH (-11.3kcal/mol), and MurA1 (-4.7kcal/mol)). Collectively, these findings suggest that I. confertiflora extracts exhibit substantial potential as antibacterial agents by targeting diverse and functionally important bacterial proteins.

Citer ce document

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0