Radiosensitizing effect of polysaccharide in Schisandrae chinensis (Turcz.) Baill. Fruit on ovarian cancer SKOV3 cells

Résumé

Purpose: To investigate the radiosensitizing effect of polysaccharide from Schisandrae Chinensis fruit (SCFP) on ovarian cancer SKOV3 cell line. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate SKOV3 cell viability. Caspase-3, caspase-9, Bax, and Bcl-2 levels in SKOV3 cells were assessed by enzyme-linked immunosorbent assay (ELISA). Furthermore, mRNA expression of caspase-3, caspase-9, Bax and Bcl-2 in SKOV3 cells were determined using quantitative real-time polymerase chain reaction (RT-qPCR) assay. Results: β-Irradiation (10 Gy) did not show obvious inhibitory effects on SKOV3 cell proliferation. However, SCFP at a dose of 1.25, 2.5 and 5 mg/mL significantly enhanced the radiosensitivity of SKOV3 cells to β-irradiation (p < 0.01). Furthermore, SCFP at concentrations of 1.25, 2.5 and 5 mg/mL significantly increased the levels of caspase-3, caspase-9 and Bax in SKOV3 cells, but decreased that of Bcl-2 (p < 0.01). In addition, SCFP combined with β-irradiation up-regulated mRNA expression of caspase-3, caspase-9 and Bax (p < 0.01), but down-regulated that of Bcl-2 in SKOV3 cells (p < 0.01). Conclusion: SCFP significantly enhances the sensitivity of nasopharyngeal carcinoma SKOV3 cells to β-irradiation. The possible mechanism of sensitization might be correlated to the induction of mitochondria-mediated apoptosis. This research provides a scientific basis for the development of SCFP for the treatment of ovarian cancer.

Citer ce document

Accès au document

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Statistiques

Consultations : 1

Téléchargements : 0