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Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Article scientifique 2016 Anglais

Résumé

AIM: . MATERIALS AND METHODS: genes amplification were performed to estimate the prevalence rate of Rickettsiae in ticks. For morphological identification of tick species, light microscope (LM) and scanning electron microscope (SEM) were used. In addition to the phylogenetic analyses of 18S rDNA, Second internal transcript spacer, 12S rDNA, cytochrome c oxidase subunit-1, and 16S rDNA were performed for molecular identification of two tick species. RESULTS: . The phylogenetic analyses of five DNA markers confirmed morphological identification by LM and SEM. The two tick species sequences analyses proved 96-100% sequences identities when compared with the reference data in Genbank records. CONCLUSION: would be useful in understanding the epidemiology of ticks and tick borne rickettsioses in Egypt.

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Abdullah, H., El-Molla, A., Salib, F., Allam, N., Ghazy, A., Abdel-Shafy, S. (2016). Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt. https://doi.org/10.14202/vetworld.2016.1087-1101

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Auteur(s)

Hend H. A. M. Abdullah Amal El-Molla Fayez A. Salib Nesreen Allam Tantawy Allam Alaa A. Ghazy Sobhy Abdel-Shafy

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