Accès ouvert

Detection of clinically relevant antibiotic resistant bacteria in shared fomites, waste water, and municipal solid wastes disposed in vicinity of residential areas of Nigerian Urban City

Article scientifique 2023 Anglais

Résumé

SStudies investigating environmental hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Nigeria are limited. This study was designed to assess various environmental sources and commonly touched surfaces as potential carriers of ARB and ARGs with implications for public health. A total of 392 samples, including sewage (36), sludge (36), diapers (20), plastics (20), water sachet polythene bags (20), food wastes (20), soil beneath dump sites (20), and frequently touched surfaces like restroom floors (80), corridors (24), door handles (56), and room floors and walls (60), were collected and screened for the presence of resistant bacteria carrying genes such as blaKPC, blaNDM-1, blaCMY-2, blaIMP, blaOXA66, and MecA. Additionally, we employed standard techniques to detect methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. We also evaluated the effectiveness of routine disinfection procedures in eliminating ARB from restroom floors. Our findings revealed that sewage, sludge, diapers, food wastes, and restroom floors are frequently contaminated with highly and moderately resistant strains of E. coli, K. pneumoniae, P. aeruginosa, and MRSA. Notably, we identified two variants of the blaOXA51-like gene (blaOXA-66 & blaOXA-180) in A. baumannii isolated from these environmental sources. Furthermore, we detected seven ESBL-K. pneumoniae, five ESBL-A. baumannii, two ESBL-E. coli, and one ESBL-P. aeruginosa, all carrying one or more ARGs (blaKPC, blaNDM-1, blaCMY-2), in isolates recovered from sewage, sludge, restroom floors, and plastics. It is worth noting that ARB persisted on restroom floors even after disinfection procedures. In conclusion, this study highlights that environmental wastes indiscriminately discarded in residential areas and shared surfaces among individuals are heavily colonized by ARB carrying ARGs of significant public health importance.

Citer ce document

Yusuf, I., Muhammad, Z., Amin, B., Shuaibu, M., Hamza, N., Isah, H., Abdullahi, N., Ene, P., Shuaibu, S., Doguwa, N., Pedro, S., Muhammad, M. (2023). Detection of clinically relevant antibiotic resistant bacteria in shared fomites, waste water, and municipal solid wastes disposed in vicinity of residential areas of Nigerian Urban City. https://doi.org/10.1099/acmi.0.000641.v2

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Ibrahim Yusuf Zainab Damji Muhammad Binta Muhammad Amin Maryam Danladi Shuaibu Nafisatu Hamza Hajara Dauda Isah Nasir Bako Abdullahi Patience James Ene Sameera Salisu Shuaibu Nasir Doguwa Shamsudeen Lekan Pedro Maryam Adamu Muhammad

Statistiques

Consultations : 1

Téléchargements : 0