A Comparison Study of Serological Test, Culturing Technique and Molecular Detection of Salmonella Typhi Among Febrile Patients in Khartoum State, Sudan

Résumé

Typhoid fever, a systemic disease caused by Salmonella enterica serovar typhi (S. typhi) it is one of the most important pathogens involved in human food-borne illness.A Cross-sectional descriptive study was conducted to detect the Salmonella typhi among patients suspected of having enteric fever using serological test, Isolation based technique and Molecular based test.The results revealed that the Widal test, isolation (Blood, Stool) of the bacteria and PCR detection showed 64%, (12%, 18%) and 27% respectively the sensitivity and specificity of different diagnostic tests were calculated using PCR as a reference method.Higher sensitivity was observed in stool culture (77.8%) and lower sensitivity was observed in Widal test (33.3%).The blood culture showed the highest specificity (97.2%), whereas Widal test showed the lowest specificity (27.4%)We suggest that the PCR technique could be used as a novel diagnostic method of typhoid fever, particularly in culture-negative cases..

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