Accès ouvert

Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Article scientifique 2016 Anglais

Résumé

BACKGROUND: Millettia macrophylla was previously reported to have estrogenic effects and to prevent postmenopausal osteoporosis in Wistar rats. So, the study deals with the identification of its secondary metabolites and the evaluation of their estrogenicity and cytotoxicity toward tumoural cells. Thus, 13 known compounds were obtained from successive chromatographic columns and identified by NMR data compared to those previously reported. METHODS: In vitro estrogenicity of the isolates and the phenolic fraction (PF) of M. macrophylla were performed by E-screen and reporter gene assays, while their cytotoxicity was evaluated by Alamar Blue (resazurin) assay. A 3-days uterotrophic assay and the ability of PF to alleviate hot flushes in ovariectomized adult rats were tested in vivo. RESULTS: μg/mL. In vivo, PF acted more efficiently than the methanol crude extract, resulting to a significant (p < 0.01) increase in the uterine wet weight, uterine protein level, uterine and vaginal epithelial height at the dose of 10 mg/kg BW. In addition, PF reduced the average duration and frequency of hot flushes induced in rat. CONCLUSION: These aforementioned results indicate that PF is a good candidate for the preparation of an improved traditional medicine able to alleviate some menopausal complaints such as vaginal dryness and hot flushes. Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth. (Fabaceae): towards a better understanding of its underlying mechanism.

Citer ce document

Zingue, S., Tchoumtchoua, J., Ntsa, D., Sandjo, L., Cisilotto, J., Ndé, C., Winter, E., Awounfack, C., Ndinteh, D., Clyne, C., Njamen, D., Halabalaki, M., Creczynski‐Pasa, T. (2016). Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms. https://doi.org/10.1186/s12906-016-1385-5

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Stéphane Zingue Job Tchoumtchoua Dieudonnée Mireille Ntsa Louis P. Sandjo Júlia Cisilotto Chantal Béatrice Magne Ndé Evelyn Winter Charline Florence Awounfack Derek Tantoh Ndinteh Colin D. Clyne Dieudonné Njamen Maria Halabalaki Tânia Beatriz Creczynski‐Pasa

Statistiques

Consultations : 2

Téléchargements : 0