An Anesthesia-Free Murine Model of Peanut Sensitization via Inhalation and Dermal Exposure for Evaluating the Immunomodulatory Effects of Dietary Arachidonic Acid v1
Résumé
Background: Developing experimental models that accurately mimic real-life exposure to peanut allergens remains a major challenge in allergy research. Many existing murine models rely on anesthesia, invasive procedures, or adjuvants, potentially limiting their physiological relevance. This study aimed to establish a non-invasive inhalation-based model of peanut exposure and to investigate the immunomodulatory effects of dietary arachidonic acid. Methods: Mice were exposed to airborne peanut flour particles under anesthesia-free conditions over a four-week sensitization period, followed by systemic challenge with peanut protein extract. The effects of dietary arachidonic acid supplementation were evaluated across different murine strains. Allergic responses were assessed using immunological and biochemical markers, including immunoglobulins, cytokines, and oxidative stress indicators in serum and lung tissues. Results: IgE remained unchanged across all groups, confirming absence of classical IgE-mediated sensitization. Measurable modulation of IgA was observed in C57BL/6 inhalation groups, and IgG1 was elevated in specific challenge-only groups. A key finding was that dietary arachidonic acid in BALB/c mice actively suppressed IL-4, IL-13, and IL-17 below naive baseline (all p<0.001), demonstrating direct anti-inflammatory activity, while groups lacking ARA supplementation showed modest Th2/Th17 cytokine elevation. Reactive oxygen species showed a consistent directional elevation in PN-exposed groups but did not reach statistical significance after Tukey correction, reflecting high inter-animal variability in the fluorescence assay. Strain-dependent differences highlighted the role of genetic background in modulating the magnitude of immune responses. Conclusions: This anesthesia-free inhalation model provides a physiologically relevant and ethically refined platform for studying peanut allergen exposure. The novel finding that dietary ARA actively suppresses Th2/Th17 cytokines below physiological baseline — without inducing IgE — reveals a previously underappreciated direct anti-inflammatory role for arachidonic acid in peanut allergen exposure, with implications for dietary immunomodulation research.
Citer ce document
Accès au document
Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter
Voir l'article sur le site de la revueAuteur(s)
Statistiques
Consultations : 1
Téléchargements : 0