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A practical DNA data storage using an expanded alphabet introducing 5-methylcytosine

Article scientifique 2025 Anglais

Résumé

The DNA molecule is a promising next-generation data storage medium. Recently, it has been theoretically proposed that non-natural or modified bases can serve as extra molecular letters to increase the information density. However, this strategy is challenging due to the difficulty in synthesizing non-natural DNA sequences and their complex structure. Here, we described a practical DNA data storage transcoding scheme named R+ based on an expanded molecular alphabet that introduces 5-methylcytosine (5mC). We demonstrated its experimental validation by encoding one representative file into several 1.3∼1.6 kbps in vitro DNA fragments for nanopore sequencing. Our results show an average data recovery rate of 98.97% and 86.91% with and without reference, respectively. Our work validates the practicability of 5mC in DNA storage systems, with a potentially wide range of applications. Availability and implementation R+ is implemented in Python and the code is available under a MIT license at https://github.com/Incpink-Liu/DNA-storage-R_plus.

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Liu, D., Xu, D., Shi, L., Zhang, J., Bi, K., Luo, B., Liu, C., Li, Y., Fan, G., Wang, W., Ping, Z. (2025). A practical DNA data storage using an expanded alphabet introducing 5-methylcytosine. https://doi.org/10.46471/gigabyte.147

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Auteur(s)

Deruilin Liu Demin Xu Liuxin Shi Jiayuan Zhang Kewei Bi Bei Luo Chen Liu Yuxiang Li Guangyi Fan Wen Wang Zhi Guang Ping

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