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Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus

Article scientifique 2017 Anglais

Résumé

Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.

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Pechnikova, E., Mishyna, M., Manukhova, T., Sokolova, O., Nikitin, N., Atabekov, J., Карпова, О. (2017). Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus. https://doi.org/10.1371/journal.pone.0183824

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Auteur(s)

Evgeniya Pechnikova Maryia Mishyna Tatiana I. Manukhova Olga S. Sokolova Nikolai A. Nikitin J.G. Atabekov О. В. Карпова

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