Accès ouvert

Metabolomic Profiling in Individuals with a Failing Kidney Allograft

Article scientifique 2017 Anglais

Résumé

BACKGROUND: Alteration of certain metabolites may play a role in the pathophysiology of renal allograft disease. METHODS: To explore metabolomic abnormalities in individuals with a failing kidney allograft, we analyzed by liquid chromatography-mass spectrometry (LC-MS/MS; for ex vivo profiling of serum and urine) and two dimensional correlated spectroscopy (2D COSY; for in vivo study of the kidney graft) 40 subjects with varying degrees of chronic allograft dysfunction stratified by tertiles of glomerular filtration rate (GFR; T1, T2, T3). Ten healthy non-allograft individuals were chosen as controls. RESULTS: LC-MS/MS analysis revealed a dose-response association between GFR and serum concentration of tryptophan, glutamine, dimethylarginine isomers (asymmetric [A]DMA and symmetric [S]DMA) and short-chain acylcarnitines (C4 and C12), (test for trend: T1-T3 = p<0.05; p = 0.01; p<0.001; p = 0.01; p = 0.01; p<0.05, respectively). The same association was found between GFR and urinary levels of histidine, DOPA, dopamine, carnosine, SDMA and ADMA (test for trend: T1-T3 = p<0.05; p<0.01; p = 0.001; p<0.05; p = 0.001; p<0.001; p<0.01, respectively). In vivo 2D COSY of the kidney allograft revealed significant reduction in the parenchymal content of choline, creatine, taurine and threonine (all: p<0.05) in individuals with lower GFR levels. CONCLUSIONS: We report an association between renal function and altered metabolomic profile in renal transplant individuals with different degrees of kidney graft function.

Citer ce document

Bassi, R., Niewczas, M., Biancone, L., Bussolino, S., Merugumala, S., Tezza, S., D’Addio, F., Nasr, M., Valderrama-Vasquez, A., Usuelli, V., Zan, V., Essawy, B., Venturini, M., Secchi, A., Cobelli, F., Lin, A., Chandraker, A., Fiorina, P. (2017). Metabolomic Profiling in Individuals with a Failing Kidney Allograft. https://doi.org/10.1371/journal.pone.0169077

Accès au document

Voir sur le dépôt source

Ce document est hébergé sur son dépôt institutionnel d'origine.

Auteur(s)

Roberto Bassi Monika A. Niewczas Luigi Biancone Stefania Bussolino Sai Merugumala Sara Tezza Francesca D’Addio Moufida Ben Nasr Alessandro Valderrama-Vasquez Vera Usuelli Valentina De Zan Basset El Essawy Massimo Venturini Antonio Secchi Francesco De Cobelli Alexander Y. Lin Anil Chandraker Paolo Fiorina

Statistiques

Consultations : 1

Téléchargements : 0